Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetMMP-1, MMP-9

General description

An excellent quenched fluorescence substrate for MMP-1 and MMP-9. Both enzymes cleave this substrate between Gly and Cys(Me), liberating a fluorescent cleavage product. Suitable for inhibitor screening and determining K_i values.

An excellent quenched fluorescence substrate for MMP-1 and MMP-9 (excitation maximum: 365 nm; emission maximum: 450 nm). Both enzymes cleave this substrate between Gly and Cys(Me), liberating a fluorescent cleavage product. Suitable for inhibitor screening and determining K_i values. Excitation max: ~365 nm; emission max: ~450 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bickett, D.M., et al. 1993. Anal. Biochem. 212, 58.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Sequence

{DNP-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(N-Me-Abz)-NH₂[Cha = β-cyclohexylalanyl; Abz = 2-aminobenzoyl (anthraniloyl)]

Warning

Toxicity: Standard Handling (A)